Four-color fluorescence in-situ hybridization is useful to assist to distinguish early stage acral and cutaneous melanomas from dysplastic junctional or compound nevus

Table 4 Comparison of the number and percentage of cases meeting each criterion of Gerami and Gaiser, and CCND1 amplification between the acral and non-acral groups of melanoma

	Acral group, n(%)	Non-acral group, n(%)	p^a value
Total	42	17
Gerami’s criteria
RREB1 > CEP6 (> 55%)	6 (14.3)	3 (17.6)	1.000
RREB1 > 2 (> 29%)	33 (78.6)	11 (64.7)	.437
MYB<CEP6 (> 40%)	19 (45.2)	5 (29.4)	.262
CCND1 > 2 (> 38%)	40 (95.2)	14 (82.4)	.274
FISH positive	41 (97.6)	15 (88.2)	.197
Gaiser’s criteria
RREB1 aberrant (> 63%)	22 (52.4)	6 (35.3)	.234
MYB<CEP6 (> 31%)	21 (50.0)	9 (52.9)	.838
MYB ≥ 2.5	21 (50.0)	7 (41.2)	.539
CCND1 ≥ 2.5	39 (92.9)	13 (76.5)	.187
FISH positive	40 (95.2)	15 (88.2)	.691
CCND1 copy numbers per cell,mean	3.74	3.03	.073
Percentage of cells with CCND1 > 2,mean	76.74%	58.67%	.007*

Abbreviations: n number
^ap values from Pearson’s chi-square test, independent t-test. *p < .05, considered statistically significant

ISSN: 1746-1596